Comparison of Biomolecular Interaction Techniques – SPR vs ITC vs MST vs BLI
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چکیده
The purpose of this white paper is to compare four techniques utilized to quantify biomolecular interactions. It provides a brief introduction to each technique, followed by tables that provide a direct comparison. The four techniques compared in this white paper are Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Microscale Thermophoresis (MST), and Biolayer Interferometry (BLI).
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MIRG Survey 2011: snapshot of rapidly evolving label-free technologies used for characterizing molecular interactions.
The field of label-free biophysical technologies used to quantitatively characterize macromolecular interactions with each other and with small molecules has grown enormously in the last 10 years. The most widely used analytical technologies for characterizing biomolecular interactions are surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), biolayer interferometry (BLI), an...
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